1jps protein structure has 19 interfacial amino acids within 5.0 Å cut-off distance. After the interfacial mutation scanning step, 361 possible mutations were built. As the result of box-whisker statistics, 28 outliers were detected within a 1.5xIQR whisker threshold. Among them 5 most enriching and 10 most depleting mutations are proposed as designer mutations.
The most depleting mutation is D52F with 3.69 ΔΔG score.
The most enriching mutation is A101L with -2.4 ΔΔG score.
The residue that is most frequently leading to a binding depleting is D52.
The residue that is most frequently leading to a binding enriching is E54.
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Position | Mutation | ΔΔG Binding Score | ΔΔG Stability Score | |
---|---|---|---|---|
Y33 | C | 2.34 | -0.09 | |
Y33 | I | 2.45 | -0.8 | |
Y33 | L | 2.34 | -0.5 | |
Y33 | V | 2.41 | -0.33 | |
D52 | F | 3.69 | -0.45 | |
D52 | I | 2.77 | -0.99 | |
D52 | L | 3.35 | -0.8 | |
D52 | V | 2.33 | -0.49 | |
D52 | Y | 3.58 | -0.7 | |
A101 | Y | 2.3 | -0.14 | |
E54 | M | -1.94 | -0.67 | |
N57 | Y | -2.16 | -0.37 | |
A101 | I | -2.37 | -1.28 | |
A101 | L | -2.4 | -1.39 | |
A101 | M | -1.96 | -0.8 | |