cluster2_1 protein structure has 29 interfacial amino acids within 5.0 Å cut-off distance. After the interfacial mutation scanning step, 551 possible mutations were built. As the result of box-whisker statistics, 33 outliers were detected within a 1.5xIQR whisker threshold. Among them 3 most enriching and 10 most depleting mutations are proposed as designer mutations.
The most depleting mutation is D58Y with 5.98 ΔΔG score.
The most enriching mutation is K212W with -2.72 ΔΔG score.
The residue that is most frequently leading to a binding depleting is Y102.
The residue that is most frequently leading to a binding enriching is S32.
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Position | Mutation | ΔΔG Binding Score | ΔΔG Stability Score | |
---|---|---|---|---|
D58 | Y | 5.98 | -0.55 | |
Y102 | R | 2.81 | -0.13 | |
D103 | A | 2.94 | -0.25 | |
D103 | C | 2.77 | -1.26 | |
D103 | F | 4.49 | -2.48 | |
D103 | K | 3.22 | -1.01 | |
D103 | P | 4.05 | -1.16 | |
D103 | R | 2.91 | -1.54 | |
D103 | W | 3.55 | -1.34 | |
D103 | Y | 5.5 | -2.42 | |
K212 | F | -1.79 | -1.18 | |
K212 | Q | -2.15 | -0.49 | |
K212 | W | -2.72 | -0.11 | |