6m0j protein structure has 39 interfacial amino acids within 7.0 Å cut-off distance. After the interfacial mutation scanning step, 741 possible mutations were built. As the result of box-whisker statistics, 197 outliers were detected within a 1.5xIQR whisker threshold. Among them 36 most enriching and 58 most depleting mutations are proposed as designer mutations.
The most depleting mutation is A475W with 5.58 ΔΔG score.
The most enriching mutation is Q493F with -2.67 ΔΔG score.
The residue that is most frequently leading to a binding depleting is Y449.
The residue that is most frequently leading to a binding enriching is Q493.
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| Position | Mutation | ΔΔG Binding Score | ΔΔG Stability Score | |
|---|---|---|---|---|
| K417 | C | 1.3 | -0.32 | |
| K417 | I | 1.22 | -1.05 | |
| K417 | L | 1.47 | -1.0 | |
| K417 | M | 1.21 | -0.47 | |
| K417 | V | 1.33 | -0.55 | |
| K417 | Y | 1.17 | -0.03 | |
| Y449 | I | 2.73 | -0.1 | |
| Y449 | L | 2.75 | -0.04 | |
| Y449 | R | 2.88 | -0.66 | |
| F456 | I | 1.5 | -0.05 | |
| F456 | L | 1.28 | -0.14 | |
| A475 | E | 1.28 | -0.92 | |
| A475 | H | 1.34 | -0.09 | |
| A475 | K | 1.1 | -0.69 | |
| A475 | R | 2.05 | -1.2 | |
| A475 | W | 5.58 | -0.5 | |
| F486 | A | 1.98 | -0.27 | |
| F486 | C | 1.6 | -0.97 | |
| F486 | D | 2.05 | -0.31 | |
| F486 | E | 2.67 | -0.67 | |
| F486 | I | 1.76 | -1.71 | |
| F486 | L | 1.0 | -1.56 | |
| F486 | N | 1.99 | -0.53 | |
| F486 | P | 1.78 | -0.67 | |
| F486 | Q | 2.72 | -0.43 | |
| F486 | R | 0.94 | -0.82 | |
| F486 | S | 2.05 | -0.3 | |
| F486 | T | 1.33 | -0.7 | |
| F486 | V | 1.19 | -1.29 | |
| F486 | W | 1.8 | -0.02 | |
| F486 | Y | 1.74 | -0.65 | |
| G496 | D | 1.15 | -0.48 | |
| G496 | F | 1.3 | -0.19 | |
| G496 | H | 3.11 | -0.11 | |
| G496 | L | 1.62 | -2.0 | |
| G496 | N | 1.4 | -0.74 | |
| G496 | Y | 1.2 | -1.09 | |
| T500 | C | 1.97 | -0.37 | |
| T500 | I | 1.65 | -1.27 | |
| T500 | L | 1.24 | -1.24 | |
| T500 | P | 1.54 | -0.69 | |
| T500 | R | 1.4 | -0.27 | |
| T500 | V | 1.86 | -0.79 | |
| T500 | W | 0.97 | -0.87 | |
| T500 | Y | 0.96 | -1.73 | |
| N501 | R | 1.93 | -0.32 | |
| G502 | D | 1.07 | -1.09 | |
| G502 | E | 1.29 | -1.46 | |
| G502 | I | 1.07 | -2.54 | |
| G502 | K | 2.59 | -1.27 | |
| G502 | N | 1.18 | -1.7 | |
| G502 | P | 3.88 | -1.62 | |
| G502 | Q | 2.23 | -0.98 | |
| G502 | R | 2.38 | -1.74 | |
| G502 | S | 1.1 | -0.89 | |
| G502 | T | 1.97 | -1.46 | |
| Y505 | I | 1.62 | -0.2 | |
| Y505 | L | 1.83 | -0.38 | |
| K417 | R | -0.62 | -0.26 | |
| G446 | R | -0.83 | -1.13 | |
| A475 | I | -0.73 | -1.61 | |
| A475 | V | -0.59 | -0.93 | |
| G476 | R | -1.24 | -0.4 | |
| N487 | C | -0.64 | -0.63 | |
| N487 | I | -0.98 | -1.36 | |
| N487 | K | -1.38 | -0.65 | |
| N487 | L | -0.53 | -1.83 | |
| N487 | M | -2.1 | -1.12 | |
| N487 | R | -0.99 | -1.33 | |
| N487 | V | -0.58 | -0.56 | |
| Q493 | F | -2.67 | -0.77 | |
| Q493 | H | -0.7 | -0.17 | |
| Q493 | I | -0.94 | -1.45 | |
| Q493 | K | -1.69 | -1.14 | |
| Q493 | L | -0.85 | -1.53 | |
| Q493 | M | -1.82 | -0.99 | |
| Q493 | R | -1.13 | -1.54 | |
| Q493 | Y | -2.19 | -1.02 | |
| G496 | R | -0.68 | -0.35 | |
| G496 | S | -1.28 | -0.51 | |
| G496 | T | -0.84 | -0.12 | |
| Q498 | E | -0.52 | -0.55 | |
| Q498 | F | -0.67 | -0.99 | |
| Q498 | I | -1.01 | -1.41 | |
| Q498 | K | -1.17 | -0.55 | |
| Q498 | L | -0.74 | -1.56 | |
| Q498 | M | -0.99 | -1.22 | |
| Q498 | N | -0.85 | -0.05 | |
| Q498 | S | -1.43 | -0.09 | |
| Q498 | T | -1.82 | -0.31 | |
| Q498 | Y | -0.64 | -1.19 | |
| N501 | L | -0.71 | -0.61 | |
| G502 | F | -0.57 | -0.41 | |
| G502 | M | -0.8 | -1.72 | |
