2nla protein structure has 26 interfacial amino acids within 5.0 Å cut-off distance. After the interfacial mutation scanning step, 494 possible mutations were built. As the result of box-whisker statistics, 34 outliers were detected within a 2.5xIQR whisker threshold. Among them 0 most enriching and 34 most Depleting Mutations are proposed as designer mutations.
The most Depleting Mutation is G262W with 82.68 ΔΔG score.
The residue that is most frequently leading to a binding depleting is G262.
The residue that is most frequently leading to a binding enriching is V321.
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| Position | Mutation | ΔΔG Protein Stability Score | Mutation Impact on Protein Folding | ΔΔG Binding Affinity Score | Mutation Impact on Protein Binding |
|---|---|---|---|---|---|
| V220 | Y | 0.4 | Negative | 5.72 | Negative |
| V253 | F | -0.24 | Positive | 4.78 | Negative |
| V253 | W | -0.64 | Positive | 6.72 | Negative |
| V253 | Y | -0.07 | Positive | 6.5 | Negative |
| D256 | P | 1.7 | Negative | 5.29 | Negative |
| G262 | A | -0.25 | Positive | 4.75 | Negative |
| G262 | C | 0.17 | Negative | 10.34 | Negative |
| G262 | D | -0.49 | Positive | 19.06 | Negative |
| G262 | E | -1.46 | Positive | 19.24 | Negative |
| G262 | F | -1.57 | Positive | 42.56 | Negative |
| G262 | H | -0.26 | Positive | 29.86 | Negative |
| G262 | I | -1.34 | Positive | 29.84 | Negative |
| G262 | K | -0.96 | Positive | 29.44 | Negative |
| G262 | L | -1.31 | Positive | 31.27 | Negative |
| G262 | M | -1.04 | Positive | 20.47 | Negative |
| G262 | N | -0.27 | Positive | 19.36 | Negative |
| G262 | P | -2.69 | Positive | 11.71 | Negative |
| G262 | Q | -0.48 | Positive | 21.32 | Negative |
| G262 | R | -0.7 | Positive | 28.16 | Negative |
| G262 | S | -0.02 | Positive | 9.29 | Negative |
| G262 | T | 0.1 | Negative | 18.31 | Negative |
| G262 | V | -0.55 | Positive | 18.63 | Negative |
| G262 | W | -1.51 | Positive | 82.68 | Negative |
| G262 | Y | -1.64 | Positive | 44.16 | Negative |
| R263 | F | -1.56 | Positive | 6.38 | Negative |
| R263 | W | 0.11 | Negative | 9.3 | Negative |
| R263 | Y | -1.29 | Positive | 7.54 | Negative |
| V265 | R | 4.7 | Negative | 6.59 | Negative |
| T266 | F | -1.4 | Positive | 13.4 | Negative |
| T266 | H | -0.39 | Positive | 6.26 | Negative |
| T266 | K | -0.74 | Positive | 5.5 | Negative |
| T266 | R | -0.39 | Positive | 6.72 | Negative |
| T266 | W | -1.94 | Positive | 19.16 | Negative |
| T266 | Y | -1.43 | Positive | 15.03 | Negative |
