1ycr protein structure has 11 interfacial amino acids within 5.0 Å cut-off distance. After the interfacial mutation scanning step, 209 possible mutations were built. As the result of box-whisker statistics, 25 outliers were detected within a 1.5xIQR whisker threshold. Among them 0 most enriching and 5 most depleting mutations are proposed as designer mutations.
The most depleting mutation is W23T with 4.42 ΔΔG score.
The residue that is most frequently leading to a binding depleting is W23.
The residue that is most frequently leading to a binding enriching is S20.
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Position | Mutation | ΔΔG Binding Score | ΔΔG Stability Score | |
---|---|---|---|---|
W23 | C | 4.35 | -0.48 | |
W23 | K | 4.16 | -0.08 | |
W23 | R | 3.93 | -0.45 | |
W23 | T | 4.42 | -0.11 | |
W23 | V | 4.05 | -0.98 | |