1ycr protein structure has 11 interfacial amino acids within 5.0 Å cut-off distance. After the interfacial mutation scanning step, 209 possible mutations were built. As the result of box-whisker statistics, 25 outliers were detected within a 1.5xIQR whisker threshold. Among them 0 most enriching and 25 most Depleting Mutations are proposed as designer mutations.
The most Depleting Mutation is F19G with 5.4 ΔΔG score.
The residue that is most frequently leading to a binding depleting is W23.
The residue that is most frequently leading to a binding enriching is S20.
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| Position | Mutation | ΔΔG Protein Stability Score | Mutation Impact on Protein Folding | ΔΔG Binding Affinity Score | Mutation Impact on Protein Binding |
|---|---|---|---|---|---|
| F19 | A | 0.88 | Negative | 4.7 | Negative |
| F19 | C | 0.16 | Negative | 4.23 | Negative |
| F19 | D | 0.87 | Negative | 5.34 | Negative |
| F19 | E | 0.44 | Negative | 4.45 | Negative |
| F19 | G | 1.51 | Negative | 5.4 | Negative |
| F19 | K | 0.53 | Negative | 4.07 | Negative |
| F19 | N | 0.31 | Negative | 4.44 | Negative |
| F19 | P | 0.2 | Negative | 5.28 | Negative |
| F19 | Q | 0.75 | Negative | 4.56 | Negative |
| F19 | S | 0.86 | Negative | 4.98 | Negative |
| F19 | T | 0.45 | Negative | 4.63 | Negative |
| W23 | A | 0.52 | Negative | 4.48 | Negative |
| W23 | C | -0.48 | Positive | 4.35 | Negative |
| W23 | D | 0.51 | Negative | 4.93 | Negative |
| W23 | E | 0.05 | Negative | 4.49 | Negative |
| W23 | G | 1.55 | Negative | 4.68 | Negative |
| W23 | H | 0.56 | Negative | 4.14 | Negative |
| W23 | K | -0.08 | Positive | 4.16 | Negative |
| W23 | N | 0.24 | Negative | 4.77 | Negative |
| W23 | P | 0.95 | Negative | 4.24 | Negative |
| W23 | Q | 0.35 | Negative | 3.9 | Negative |
| W23 | R | -0.45 | Positive | 3.93 | Negative |
| W23 | S | 0.51 | Negative | 4.61 | Negative |
| W23 | T | -0.11 | Positive | 4.42 | Negative |
| W23 | V | -0.98 | Positive | 4.05 | Negative |
