4G6M-matched protein structure has 21 interfacial amino acids within 5.0 Å cut-off distance. After the interfacial mutation scanning step, 399 possible mutations were built. As the result of box-whisker statistics, 12 outliers were detected within a 1.5xIQR whisker threshold. Among them 0 most enriching and 5 most depleting mutations are proposed as designer mutations.
The most depleting mutation is D58Y with 5.11 ΔΔG score.
The residue that is most frequently leading to a binding depleting is Y102.
The residue that is most frequently leading to a binding enriching is S150.
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Position | Mutation | ΔΔG Binding Score | ΔΔG Stability Score | |
---|---|---|---|---|
D58 | F | 3.31 | -0.35 | |
D58 | Y | 5.11 | -0.65 | |
Y102 | R | 3.28 | -0.04 | |
D103 | R | 3.51 | -1.49 | |
D103 | Y | 4.23 | -2.38 | |